FIG. 6.

Colocalization of Us9 with envelope proteins gB and gC. PK15 cells grown on glass coverslips were infected with PRV Be at an MOI of 10. The cells were fixed and stained for Us9 and gB (A, B, and C) at 6 h postinfection and for Us9 and gC (D, E, and F) at 4 h post-infection. Us9 (A and D) was detected with an indocarbocyanine-conjugated (Cy3) donkey anti-rabbit secondary antibody. gB (B) and gC (E) were visualized with a fluorescein isothiocyanate-conjugated donkey anti-goat secondary antibody. The Us9 (red) and gB (green) colocalization is shown in panel C. The Us9 (red) and gC (green) colocalization is shown in panel F.