FIG. 4.

Deletion analysis of IVT E1 binding to GST-E2. In vitro association of bead-bound GST-E2 to radiolabelled IVT E1 N- and C-terminal deletion mutants was assayed as described in Materials and Methods. Autoradiographs show E1 protein that bound to and then was eluted from the beads. Input IVT protein is denoted I. (A) Binding of selected IVT E1 N-terminal deletion mutants to GST-E2 at 20°C. (B) Binding of selected IVT E1 C-terminal deletion mutants to GST-E2 at 20°C. This reciprocal binding experiment confirms the deletion analysis data obtained with IVT E2 and GST-E1 at 20°C. The positions of molecular mass markers are shown to the left of each gel.