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Competitive binding of E2 and p68 to E1. Competition between IVT p68 and GST-E2 for binding to GST-E1 was assayed as described in Materials and Methods. The results demonstrate that GST-E2 and p68 compete for E1 binding with p68 in a dose-dependent manner, compared to GST-TBP and GST-E1Δ597N control proteins. Autoradiograms were scanned densitometrically to quantify the data.
