. 2024 Feb 23;15:1346442. doi: 10.3389/fmicb.2024.1346442

Table 5.

Comparison between Carba-Screen results and NGS resistome analysis related to K. pneumoniae (Kp) isolates.

						Realquality carbascreen screening (1° level)			Realquality carbascreen identification (2° level)						NGS (resistome analysis related to β-lactams resistance)
						Class B	Class A + D	Acinetobacter OXA	Carba B			Carba A + D			NGS (resistome analysis related to β-lactams resistance)
Strain	Carbapenem-resistant phenotype	Identification	MLST	k-locus	o-locus	Class B	Class A + D	Acinetobacter OXA	NDM	VIM	IMP	KPC	OXA-48	mcr	bla_NDM	bla_KPC	bla_SHV				bla_CTX	bla_TEM		bla_OXA		LamB mutants	ompK35/36/37 mutants
Kp 229	Positive	K. pneumoniae	ST395	2	O1/O2v1	Positive	Positive	Negative	bla_NDM	Negative	Negative	bla_KPC	Negative	Negative	NDM-1				SHV-187		CTX-M-15			OXA-1		Yes	Yes (ompK36, ompK37)
Kp 322	Positive	K. pneumoniae	ST395	2	O1/O2v1	Positive	Positive	Negative	bla_NDM	Negative	Negative	bla_KPC	Negative	Negative	NDM-1				SHV-187		CTX-M-15			OXA-1		Yes	Yes (ompK36, ompK37)
Kp 326	Positive	K. pneumoniae	ST395	2	O1/O2v1	Positive	Positive	Negative	bla_NDM	Negative	Negative	bla_KPC	Negative	Negative	NDM-1		SHV-61	SHV-152			CTX-M-15			OXA-1		Yes	Yes (ompK36, ompK37)
Kp 233	Positive	K. pneumoniae	ST395	2	O1/O2v1	Positive	Negative	Negative	bla_NDM	Negative	Negative	Negative	Negative	Negative	NDM-1				SHV-187		CTX-M-15			OXA-1		Yes	Yes (ompK36, ompK37)
Kp 236	Positive	K. pneumoniae	ST395	2	O1/O2v1	Positive	Negative	Negative	bla_NDM	Negative	Negative	Negative	Negative	Negative	NDM-1				SHV-187		CTX-M-15			OXA-1		Yes	Yes (ompK36, ompK37)
Kp 273	Positive	K. pneumoniae	ST395	2	O1/O2v1	Positive	Negative	Positive	bla_NDM	Negative	Negative	Negative	Negative	Negative	NDM-1						CTX-M-15	TEM-181		OXA-1		No	No
Kp 304	Positive	K. pneumoniae	ST395	2	O1/O2v1	Positive	Negative	Positive	bla_NDM	Negative	Negative	Negative	Negative	Negative	NDM-1						CTX-M-15		TEM-122	OXA-1		No	Yes (ompK36)
Kp 281	Positive	K. pneumoniae	ST395	2	O1/O2v1	Positive	Negative	Positive	bla_NDM	Negative	Negative	Negative	Negative	Negative	NDM-1						CTX-M-15	TEM-181		OXA-1		Yes	Yes (ompK36)
Kp 267	Positive	K. pneumoniae	ST395	2	O1/O2v1	Positive	Negative	Positive	bla_NDM	Negative	Negative	Negative	Negative	Negative	NDM-1			SHV-152			CTX-M-15	TEM-181		OXA-1		Yes	Yes (ompK35)
Kp 300	Positive	K. pneumoniae	ST395	2	O1/O2v1	Positive	Negative	Positive	bla_NDM	bla_VIM	Negative	Negative	Negative	Negative	NDM-1						CTX-M-15	TEM-181		OXA-1		Yes	Yes (ompK36)
Kp 234	Positive	K. pneumoniae	ST17	25	O5	Positive	Positive	Negative	Negative	bla_VIM	Negative	bla_KPC	Negative	Negative											OXA-9	No	Yes (ompK37)
Kp 241	Positive	K. pneumoniae	ST17	25	O5	Negative	Positive	Negative	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3	SHV-61	SHV-152	SHV-187						OXA-9	No	No
Kp 325	Positive	K. pneumoniae	ST17	25	O5	Negative	Positive	Negative	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3		SHV-152				TEM-181				Yes	Yes (ompK36)
Kp 324	Positive	K. pneumoniae	ST17	25	O5	Positive	Positive	Negative	Negative	bla_VIM	Negative	bla_KPC	Negative	Negative		KPC-3						TEM-181	TEM-122		OXA-9	No	Yes (ompK37)
Kp 323	Positive	K. pneumoniae	ST17	25	O5	Negative	Positive	Negative	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3			SHV-187			TEM-181			OXA-9	No	Yes (ompK36)
Kp 209	Positive	K. pneumoniae	ST101	17	O1/O2v1	Negative	Positive	Negative	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3			SHV-187	SHV-212						No	Yes (ompK36, ompK37)
Kp 244	Positive	K. pneumoniae	ST101	17	O1/O2v1	Positive	Positive	Negative	bla_NDM	Negative	Negative	bla_KPC	Negative	Negative		KPC-3			SHV-187	SHV-212						Yes	Yes (ompK36, ompK37)
Kp 230	Positive	K. pneumoniae	ST101	17	O1/O2v1	Negative	Positive	Negative	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3			SHV-187	SHV-212						No	Yes (ompK36)
Kp 312	Positive	K. pneumoniae	ST101	17	O1/O2v1	Negative	Positive	Positive	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3						TEM-181			OXA-9	No	No
Kp 316	Positive	K. pneumoniae	ST307	102	O1/O2v2	Negative	Positive	Positive	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3						TEM-181		OXA-1		No	No
Kp 341	Positive	K. pneumoniae	ST307	102	O1/O2v2	Negative	Positive	Negative	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3					CTX-M-15		TEM-122	OXA-1	OXA-9	No	No
Kp 285	Positive	K. pneumoniae	ST307	102	O1/O2v2	Negative	Positive	Negative	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3					CTX-M-15	TEM-181	TEM-122	OXA-1	OXA-9	No	No
Kp 327	Positive	K. pneumoniae	ST46	64	O1/O2v1	Negative	Positive	Negative	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3										No	Yes (ompK37)
Kp 248	Positive	K. pneumoniae	ST46	64	O1/O2v1	Negative	Positive	Negative	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3	SHV-61	SHV-152				TEM-181			OXA-9	No	Yes (ompK36, ompK37)
Kp 232	Positive	K. pneumoniae	ST35	22	O1/O2v1	Negative	Positive	Negative	Negative	Negative	Negative	bla_KPC	Negative	Negative		KPC-3			SHV-187			TEM-181				No	Yes (ompK36, ompK37)