Table 1.
Primers used in conventional PCR performed in this study.
| Gene | Primer | Sequence (5′ → 3′) | Annealing temperature (°C) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|
| eae | eae-common-F | CCCGAATTCGGCACAAGCATAAGC | 55 | 881 | (30) |
| eae-common-R | CCCGGATCCGTCTCGCCAGTATTCG | ||||
| Stx1 | EC-vt1_2-F | CGTCTTTACTGATGATTGATAGTGGC | 58 | 637 | (31) |
| EC-vt1_2-R | CGCGATGCATGATGATGAC | ||||
| Stx2 | EC-vt2_2-F | TACCACTCTGCAACGTGTCG | 58 | 297 | (31) |
| EC-vt2_2-R | CGATACTCCGGAAGCACATT | ||||
| papC | pap1 | GACGGCTGTACTGCAGGGTGTGGCG | 55 | 328 | (32) |
| pap2 | ATATCCTTTCTGCAGGGATGCAATA | ||||
| hlyA | hlyA-F | AACAAGGATAAGCACTGTTCTGGCT | 55 | 1,177 | (33) |
| hlyA-R | ACCATATAAGCGGTCATTCCCGTCA | ||||
| cnf1 | cnf1-A | GAACTTATTAAGGATAGT | 54 | 543 | (32) |
| cnf1-B | CATTATTTATAACGCTG |