Table 1. Experimental Groupsa.
| group | antigen or control | antigen dose (μg) | infection |
|---|---|---|---|
| 1 | saline | no | |
| 2 | saline | yes | |
| 3 | empty LNPs | yes | |
| 4 | Tc24 mRNA | 10 | yes |
| 5 | APS-2 mRNA | 10 | yes |
| 6 | Tc24 + ASP-2 mRNA | 20 (10 each) | yes |
All mice were infected with 5000 T. cruzi H1 clones K68 by intraperitoneal injection. Mice received immunization subcutaneously, the prime at 70 and two boosters at days 77 and 84 DPI.