FIG. 12.

GuHCl extraction analysis of purified B capsids lacking individual cleavage and packaging proteins. Capsids from Vero cells infected with KOS, hr74 (UL6⁻), hr81-1 (UL15⁻), KUL25NS (UL25⁻), gCB (UL28⁻), or hr64 (UL32⁻) were purified from sucrose gradient and either mock treated or treated with 2.0 M GuHCl. In this experiment, twice as much of the GuHCl-treated capsid extract as the untreated extract was loaded in each lane. (A) A silver-stained SDS-PAGE gel shows capsid structural proteins. Assignment of bands was based on previous reports (29, 44). The molecular masses of protein size markers (in kilodaltons) are given on the left. (B) Immunoblot analysis for the presence of UL15, UL25, and UL28. A long (20 min) exposure of the UL15 immunoblot is shown.