FIG. 2.

Analysis of total-cell lysates from cells infected with wild-type and mutant viruses defective in individual DNA cleavage and packaging genes. Vero cells were either mock infected or infected with wild-type KOS virus or mutant UL6 (hr74), UL15 (hr81-1 and hr81-2), UL25 (KUL25NS), UL28 (gCB), or UL32 (hr64) virus at an MOI of 10 PFU per cell for 16 h. Total-cell lysates were examined for the expression of individual proteins (except UL6) as indicated by ECL immunoblotting. All immunoblottings for UL6 in this paper were carried out by alkaline phosphatase color development as described in Materials and Methods.