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Plasmid with selected primer pairs. (A) For an identity PCR for a non-FLEx/DIO construct, choose forward (fwd) and reverse (rev) primers that target your insert between the ITRs. (B) For an identity PCR for a FLEx/DIO construct, choose one primer pair that targets your insert area (fwd2 + rev2), one primer pair with one primer being outside of and one in between the lox-sites (fwd3 + rev2) and one control primer pair (fwd3 + fwd 2).
