Fig. 1.

Three-dimensional images of ER after injury. a After reconstructing ER from serial images with FIB/SEM, the lamella-like structures (arrowheads) are detected around the nucleus (N) in the cell body. On the other hand, the distribution is sparse around the area close to the plasma membrane (PM) (blue dotted line). b Higher magnification three-dimensional images of ER around the nucleus in Fig. 1(a). c Higher magnification of Fig. 1(a) shows the three-dimensional images of ER close to the PM (blue dotted line). d In the injured cell at one week after injury, the ER-lamella structures are collapsed, and ER is distributed uniformly inside the neuron. The structure of the ER looks mesh-like structure throughout the cells (N: nucleus, blue dotted line: PM). e Higher magnification 3D images of the ER around the nucleus in Fig. 1(d). f Higher magnification 3D images of the ER form close to the PM (blue dotted line) in Fig. 1(d). Scale bar a, d 2 μm; b, c, e, f 1 μm