Fig. 3.

ER branching points analysis in the control and injured motor neuron. a Representative cross-sectional images of FIB/SEM. The yellow landmarks indicate the branching points. b The 3D unit volume (1 µm³) of the ER branching points analysis contains some branching points. c The branching points of the ER perinucleus and ER periphery are markedly increased in the injured motor neurons. Ten ER from each perinuclear and peripheral region in each cell (control: n = 3 neurons of three mice; injured: n = 3 neurons of three mice) were measured. The error bars show the standard deviation. The p-value was determined using the Two-way ANOVA with post hoc Tukey’s test: ***p < 0.001. Scale bar 500 nm