Fig. 6.

E-Syt1 accumulation observed along PM after axon injury. a Almost weak expression of E-Syt1 was detected in the control motor neurons. b E-Syt1 expression was detected along the PM of the injured motor neurons. c–f The transition in E-Syt1 expression after injury was observed from 3 to 28 dpi, the expression observed at 3 dpi until reach its peak at 7 dpi then little by little decreased. a′–f′ Fluorescent intensities of E-Syt1 expression along the indicated lines were assessed in motor neurons before and after hypoglossal nerve transection. g qRT-PCR analysis was conducted on E-Syts mRNA extracted from both the control and injured sides of fourteen WT mice 7 days after surgery. The results have been normalised to the housekeeping gene GAPDH, and the values are presented as mean ± SD. The statistical analysis showed significant differences (***p < 0.0001) based on a two-way ANOVA with a post hoc Tukey’s test. h–j Representative image for the injured motor neuron (E-Syt1) surrounded by microglia (Iba1) which determined the boundaries of the motor neuron. Dotted red lines for the intensity fluorescent whilst dotted white circle determine the nucleus. Scale bar 10 µm