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. 2024 Apr 5;15:2939. doi: 10.1038/s41467-024-47317-1

Fig. 1. CD31+Emcn+ vessels appear in sclerotic endplates in LSI and aged mice.

Fig. 1

Representative immunofluorescent images of CD31+ (green), Emcn+ (red) cells and DAPI (blue) staining of nuclei in the caudal endplates of L4/5 at 4 and 8 weeks after LSI surgery or sham surgery (a) or in 3-month-old or 20-month-old mice (b). Scale bars, 50 μm. c, d Permillage of CD31+Emcn+ area of a and b. Representative three-dimensional µCT images of the caudal endplates of L4/5 (coronal view) at 4 and 8 weeks after LSI surgery or sham surgery mice (e) or in 3-month-old or 20-month-old mice (f). Scale bars, 500 μm. g, h Quantitative analysis of the total porosity of e and f. Representative images of safranin O and fast green staining of endplate sections at 4 and 8 weeks after LSI or sham surgery (i) or in 3-month-old or 20-month-old mice (j), proteoglycan (red) and cavities (green). Scale bars, 50 μm. k, l Endplate scores as an indication of endplate degeneration based on safranin O and fast green staining. Representative immunohistochemical images of Osterix (Osx, top) or Osteocalcin (Ocn, bottom) in the endplates at 4 weeks or 8 weeks after LSI or sham surgery (m) or in 3-month-old or 20-month-old mice (n). Scale bars, 50 μm. o–r Quantitative analysis of the number of Osx+ cells or Ocn+ cells of m and n. n = 6 per group. Data are represented as means ± standard deviations, as determined by two-tailed Student’s t test or One-way ANOVA. Source data are provided as a Source Data file.