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. 2024 Apr 5;15:2939. doi: 10.1038/s41467-024-47317-1

Fig. 10. IL-10 stimulates pSTAT3 signaling to induce angiogenesis.

Fig. 10

a mRNA expression of several key angiogenesis-associated factors, including Vegfa (VEGFA), Mmp2 (MMP2), Pdgfb (PDGFB), Mmp9 (MMP9), Fgf2 (FGF2), Hif1a (HIF-1α), and Cdh5 (VEC) in endothelial cells treated with 50 ng/ml IL-10 or vehicle for 24 h, determined by qRT-PCR. n = 5 per group. b–g Endothelial cells were treated with 50 ng/ml IL-10 or vehicle for 24 h, plus 5 μmol/L nonpeptidic selective Stat3 inhibitor (Stattic) for 24 h. b Western blots of the phosphorylation of STAT3 (pSTAT3), STAT3, VEGFA, MMP2, and PDGFB in endothelial cells. c Quantitative analysis of b. n = 3 per group. d Representive tube formation images of endothelial cells. Scale bars, 50 μm. e Quantitative analysis of d. n = 5 per group. f Predicated pSTAT3 binding sites on Vegfa, Pdgfb, and Mmp2 promoters and sequences of specific primers. g ChIP analysis of pSTAT3 binding on specific Vegfa, Pdgfb, and Mmp2 promoters in endothelial cells with vehicle or IL-10 treatment. n = 3 per group. Data are represented as means ± standard deviations, as determined by two-tailed Student’s t test or One-way ANOVA. Source data are provided as a Source Data file.