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. 2024 Apr 5;15:2939. doi: 10.1038/s41467-024-47317-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Representative immunofluorescent images of tdTom⁺ (red), F4/80⁺ (green) cells and DAPI (blue) staining of nuclei in LSI surgery model (a) or aging model (b) of cdkn2a (p16)^tdTom mice. Scale bars, 50 μm. c, d Quantitative analysis of the number of F4/80⁺tdTom⁺ cells in the endplates of a and b. n = 6 per group. e Representative images of flow cytometry analysis. cdkn2a (p16)^tdTom mice underwent sham or LSI surgery. Cells were isolated from the endplates. f Quantitative analysis of e. n = 3 per group. g Representative images of senescence-associated distension of satellites (SADS, green), F4/80 (red) and DAPI (blue) staining of nuclei in the endplates of LSI surgery mice or aged mice by performing simultaneous immunofluorescent staining and immune-fluorescent in situ hybridization (FISH). Scale bars, 10 μm. h Quantitative analysis of the number of F4/80⁺SADS⁺ cells (≥4 SADS/cell) in endplates of g. n = 6 per group. i Representative immunofluorescent images of γH2A.X (green), F4/80 (red) and DAPI (blue) staining of nuclei in the endplates of LSI surgery mice or aged mice. Scale bars, 10 μm. j Quantitative analysis of the number of F4/80⁺γH2A.X⁺ cells in the endplates of i. n = 6 per group. Representative immunofluorescent images of F4/80 (red), CD31 (green) and DAPI (blue) staining of nuclei in the endplates at 4, and 8 weeks after LSI or sham surgery (k) or in 3-month-old or 20-month-old mice (l). Scale bars, 50 μm. n = 3–6 per group. Data are represented as means ± standard deviations, as determined by two-tailed Student’s t test or One-way ANOVA. Source data are provided as a Source Data file.