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. 2024 Apr 5;15:2939. doi: 10.1038/s41467-024-47317-1

Fig. 7. Deletion of macrophages inhibits LSI-induced endplate sclerosis.

Fig. 7

a Representative immunofluorescent images of F4/80+ (red) cells and DAPI (blue) staining of nuclei in mouse caudal endplates of L4/5 in iDTRΔLyz2 mice with diphtheria toxin (DT) or vehicle injection at 8 weeks after LSI surgery. Scale bars, 50 μm. b–i All the experiments were conducted at 8 weeks after LSI or sham surgery in iDTRΔLyz2 mice with DT or vehicle injection. b Representative images of immunofluorescent analysis of Emcn+ (red), CD31+ (green) cells and DAPI (blue) staining of nuclei in the endplates. Scale bars, 50 μm. c Permillage of CD31+Emcn+ area in the endplates of b. d Representative µCT images of the caudal endplates of L4/5 (coronal view). Scale bars, 500 μm. e Quantitative analysis of the total porosity of d. f Representative images of safranin O and fast green staining of the endplates. Scale bars, 50 μm. g Endplate scores of f. h Representative immunohistochemical images of Osterix (Osx) in the endplates. Scale bars, 50 μm. i Quantitative analysis of the number of Osx+ cells in the endplates of h. n = 6 per group. Data are represented as means ± standard deviations, as determined by One-way ANOVA. Source data are provided as a Source Data file.