Figure S5. Human transgenic DEFA1A3 copy numbers associate with reduced Toll-like receptor induction after uropathogenic E. coli; CFT073 superinfection.
Screening of Toll-like receptor superfamily was performed via RT–qPCR from DEFA1A3-carrier and non-carrier infected kidneys with 0, 4, and 8 human gene copies. (A, B, D, F) After uropathogenic E. coli superinfection, mRNA expression differences for (A) Tlr2, (B) Tlr4, (D) Tlr6, and (F) Tlr9 gene targets at 6 hpi between DEFA0/0 and DEFA4/4 mice are represented as the mean ± SD. (C, E) No significant differences for (C) Tlr5 and (E) Tlr7 expression across groups. Statistical differences between groups with three to five biological replicates were measured using one-way ANOVA with Tukey’s post hoc test.