TABLE 1.
MDEV derived from the molecular clone displays reciprocal interference with a biological isolatea
| LAPSN pseudotype | LAPSN titer (AP+ FFU/ml) on cell type:
|
||
|---|---|---|---|
| dunni/N2 | dunni/N2 + activated MDEV | dunni/N2 + cloned MDEV | |
| PA317 | 106 | 106 | 106 |
| Activated MDEV | 8 × 104 | <10 | <10 |
| Cloned MDEV | 4 × 104 | <10 | <10 |
a
Uninfected dunni/N2 cells and dunni/N2 cells that had been previously infected with either MDEV activated from M. dunni cells or MDEV derived from the pMDEV molecular clone were plated at a density of 2 × 105 cells per 3.5-cm-diameter well on day 1. The cells were exposed to LAPSN with the indicated pseudotypes on day 2 and were stained for AP+ foci on day 4. Values are expressed as the means of duplicates which varied by no more than 20% from the mean. The experiment was repeated by plating 105 cells per 3.5-cm-diameter well, with nearly identical results being achieved.