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. 2024 Mar 27;10(7):e28726. doi: 10.1016/j.heliyon.2024.e28726

Fig. 2.

Fig. 2

Design and screening of RPA primers. (A) Map of nucleotide variation in the UL75 gDNA region of the 103 HCMV strains and location of the target region for RPA. (B) Conservation of target region for RPA and crRNAs among all 103 HCMV strains. (C) Gel electrophoresis image of RPA product amplified with two different primer sets. RPA reactions were performed for the indicated time. The original agarose gel image is provided in the supplementary data.