Fig. 4.

LoD determination of RPA-CRISPR-Cas12a HCMV diagnosis platform. RPA-CRISPR-Cas12a reactions were performed using serially diluted UL75 plasmid. (A) Fluorescence signals were detected during the CRISPR-Cas12a reaction by a plate reader every minute. (B) Fluorescence values after 30 min of the CRISPR-Cas12a reactions in panel A. Error bars indicate SD (n = 3). (C) Diagnosis results were read out using LFA strips after 30 min of Cas12a reaction. The photo was taken 3 min after the strips were dipped. (D) Relative band intensity of LFA strips in panel C. Error bars indicate SD (n = 3). Comparisons between groups were performed using the two-tailed unpaired Student's t-test. N: negative control, no template sample.