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Specificity of RPA-CRISPR-Cas12a HCMV diagnosis platform. RPA-CRISPR-Cas12a reactions were performed using viral DNA of HSV-1, HSV-2, VZV, and HCMV (A). Diagnosis results were read out using LFA after 30 min of CRISPR-Cas12a reaction. (B) Relative band intensity of the LFA strips in panel A. Error bars represent the results of two independent experiments. N: negative control, no template sample.
