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. 2024 Mar 27;10(7):e28726. doi: 10.1016/j.heliyon.2024.e28726

Table 2.

Clinical validation of HCMV-RPA-CRISPR diagnosis. PPA: Positive Prediction Agreement, NPA: Negative Prediction Agreement.

RPA-CRISPR-Cas12a qPCR diagnosis
PPA NPA
Positive Negative
Fluorescence Positive 4 0 4 of 4 = 100% 9 of 9 = 100%
Negative 0 9
LFA Positive 4 0 4 of 4 = 100% 9 of 9 = 100%
Negative 0 9