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2iLIF + Bsd medium
| Reagent | Final concentration | Amount |
|---|---|---|
| N2B27 medium | N/A | 50 mL |
| PD0325901 (10 mM) | 1 μM | 5 μL |
| murine LIF (200 mg/ mL) | 20 ng/mL | 5 μL |
| CHIR99021 (10 mM) | 3 μM | 15 μL |
| Blasticidin-S-HCL (10 mg/mL) | 20 μg/mL | 100 μL |
| Total | N/A | 50 mL |
Store medium at 4°C and use within 1 week.
