FACS buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| N2B27 medium | N/A | 10 mL |
| Penicillin-Streptomycin (10,000 U/mL) | 100 U/mL | 100 μL |
| Primocin (50 mg/mL) | 50 μg/mL | 10 μL |
| Total | N/A | 10 mL |
Make fresh medium immediately before experiment. Do not store FACS buffer.
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.