IVC medium (Amadei, G. et al.)⁵

Reagent	Final concentration	Amount
advanced DMEM/F12	N/A	33.485 mL
FBS	30%	15 mL
GlutaMAX (100 ×)	1×	500 μL
ITS-X (100 ×)	1×	500 μL
β-estradiol (80 μM)	8 nM	5 μL
Progesterone (2 mg/mL)	200 ng/mL	5 μL
N-acetyl-L-cysteine (250 mM)	25 μM	5 μL
3,3′,5-Triiodo-L-thyronine sodium salt (1 mM)	100 nM	5 μL
Penicillin-Streptomycin (10,000 U/mL)	100 U/mL	500 μL
Total	N/A	50 mL

Store medium at 4°C and use within 1 week.