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In vitro transcription-permissive fractions contain PPBP. Labeled polh B domain was incubated either alone (lane 1); with 1 μg of crude Sf9 cell nuclear extract (lane 2); or with 0.3 (lane 3), 0.5 (lane 4), 0.75 (lane 5), or 1.0 (lane 6) M KCl phosphocellulose fractions.
