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Expression of Galα1-3Gal on cultured cells. Cells used in these experiments were stained with purified anti-Gal Ab and analyzed by flow cytometry as described in Materials and Methods. Peaks depict Galα1-3Gal expression on the surfaces of L-929, N115, HeLa, BHK-21, SK-N-MC (SK) cells or SK-N-MC cells transduced with recombinant GT (SK-GT), and cells from GT-deficient (GT-KO) or GT-sufficient (GT-WT) mice.
