TABLE 1.
Inactivation of LCMV by NHSa
| Expt | Virus | Inhibitor | Log10 PFU after treatment
|
||
|---|---|---|---|---|---|
| NHS | Heat-inactivated NHS | MEM | |||
| 1 | LCMV-BHK | 4.0 | 3.9 | 4.2 | |
| LCMV-HeLa | 5.0 | 4.9 | 4.8 | ||
| LCMV-L-929 | 1.5 | 3.9 | 4.2 | ||
| LCMV-SK | 4.3 | 4.5 | 4.5 | ||
| LCMV-SK-GT | 2.4 | 4.0 | 4.2 | ||
| 2 | LCMV-L-929 | HBSS | <1 | 3.7 | |
| Galα1-3Gal | 2.5 | 3.9 | |||
| Sucrose | <1 | 3.7 | |||
| LCMV-SK | HBSS | 3.9 | 4.3 | ||
| Galα1-3Gal | 4.1 | 4.2 | |||
| Sucrose | 3.9 | 4.0 | |||
| LCMV-SK-GT | HBSS | 2.0 | 3.8 | ||
| Galα1-3Gal | 3.3 | 3.7 | |||
| Sucrose | 2.0 | 3.7 | |||
| 3 | LCMV-GT-WT | 1.6 | 4.3 | 4.0 | |
| LCMV-GT-KO | 3.4 | 3.6 | 3.4 | ||
| LCMV-SK | 4.2 | 4.2 | 4.1 | ||
| LCMV-SK-GT | 2.1 | 4.0 | 3.7 | ||
a
LCMV passaged in different cell lines was treated with fresh or heat-inactivated NHS or with MEM for 1 h at 37°C and titrated for PFU. In expt 2, either HBSS, soluble Galα1-3Gal, or sucrose was included in the inactivation assay.