TABLE 2.
Replication of LCMV in mice containing Anti-Gal Aba
| Virus | Dose | Log10 PFU/spleen (mean ± SD)
|
|
|---|---|---|---|
| GT-sufficient (Ab−) mice | GT-deficient (Ab+) mice | ||
| LCMV-SK | 5 × 104 | 6.5 ± 0.15 | 6.1 ± 0.19 |
| LCMV-SK-GT | 5 × 104 | 5.7 ± 0.03 | 5.5 ± 0.06 |
| LCMV-SK | 5 × 102 | 5.2 ± 0.13 | 4.6 ± 0.03 |
| LCMV-SK-GT | 5 × 102 | 4.0 ± 0.13 | 3.4 ± 0.01 |
a
Mice were immunized with sheep erythrocyte membranes to induce Ab to Galα1-3Gal. No Abs were detected in immunized GT-sufficient 129 WT mice, but high titers were elicited in GT-deficient (GT-KO) mice, which recognize Galα1-3Gal as a foreign antigen. Mice were inoculated intravenously with 5 × 104 (n = 4 per group) or 5 × 102 (n = 2 per group) PFU of LCMV passaged in SK or SK-GT cells and titrated for PFU/spleen at 3 days postinfection.