TABLE 3.
Inactivation of LCMV with immune mouse seruma
| Expt | Virus | Treatment | Log10 PFU
|
|
|---|---|---|---|---|
| Direct assay | PEC yield | |||
| 1 | LCMV-SK | NHS | 3.7 | 3.5 |
| MEM | 3.8 | 3.3 | ||
| GT-def 1 (Ab+) | 3.8 | 3.5 | ||
| GT-def 2 (Ab+) | 3.8 | 3.6 | ||
| GT-suf (Ab−) | 3.7 | 3.5 | ||
| LCMV-SK-GT | NHS | 1.7 | <1 | |
| MEM | 3.0 | 2.1 | ||
| GT-def 1 (Ab+) | 2.5 | 2.5 | ||
| GT-def 2 (Ab+) | 2.5 | 2.7 | ||
| GT-suf (Ab−) | 3.3 | 2.8 | ||
| 2 | LCMV-SK | NHS | 3.9 | 2.0 |
| ΔNHS | 4.0 | 2.3 | ||
| MEM | 4.2 | 2.4 | ||
| GT-def (Ab+) | 4.1 | 2.7 | ||
| GT-suf (Ab−) | 4.0 | 3.2 | ||
| LCMV-SK-GT | NHS | 2.3 | <1 | |
| ΔNHS | 3.8 | 2.3 | ||
| MEM | 3.9 | 2.6 | ||
| GT-def (Ab+) | 3.1 | 2.1 | ||
| GT-suf (Ab−) | 3.8 | 2.4 | ||
a
LCMV passaged in different cell lines was treated with NHS, heat-inactivated NHS (ΔNHS), MEM, or serum from GT-sufficient 129 WT (GT-suf) or GT-deficient (GT-def) mice. All mice had been previously immunized against sheep erythrocytes, but only the GT-deficient mice developed anti-Gal Ab responses. Samples were either titrated directly on Vero cells or used to infect 129 mouse PEC. Virus released into the PEC culture fluid 1 day postinfection was then titrated for PFU on Vero cells.