TABLE 4.
Inactivation of heterologous viruses by NHSa
| Expt | Virus | Log10 PFU after treatment
|
||
|---|---|---|---|---|
| NHS | Heat-inactivated NHS | MEM | ||
| 1 | HSV-SK | <1 | <1 | 3.5 |
| HSV-SK-GT | <1 | <1 | >3.7 | |
| SV-SK | 3.2 | 3.5 | 3.6 | |
| SV-SK-GT | 3.4 | 3.3 | 3.3 | |
| VSV-SK | 2.2 | 3.6 | 4.1 | |
| VSV-SK-GT | 2.0 | 4.0 | 3.9 | |
| 2 | NDV-SK | 1.8 | 3.8 | 3.7 |
| NDV-SK-GT | <1 | 3.9 | 4.2 | |
| SV-SK | 3.4 | 3.6 | 3.5 | |
| SV-SK-GT | 3.5 | 3.4 | 3.7 | |
| VSV-SK | 2.2 | 3.6 | 4.3 | |
| VSV-SK-GT | 1.8 | 3.9 | 4.0 | |
| 3 | NDV-SK-STOCK 1 | <1 | 3.0 | 3.1 |
| NDV-SK-STOCK 2 | 1.7 | 4.0 | 4.1 | |
| NDV-SK-GT-STOCK 1 | <1 | 3.4 | 3.4 | |
| NDV-SK-GT-STOCK 2 | 1.5 | 4.1 | 4.2 | |
| 4 | SV-GT-WT | 2.9 | 4.1 | 4.2 |
| SV-GT-KO | 4.2 | 4.4 | 4.4 | |
| VSV-GT-WT | <1 | 4.0 | 4.1 | |
| VSV-GT-KO | 1.0 | 4.0 | 4.1 | |
| 5 | SV-GT-WT | 3.5 | 4.0 | 4.3 |
| SV-GT-WT + nanase | 3.4 | 4.3 | 4.4 | |
| SV-GT-KO | 4.2 | 4.3 | 4.4 | |
| SV-GT-KO + nanase | 3.8 | 4.1 | 4.2 | |
| SV-SK | 3.9 | 3.8 | 3.8 | |
| SV-SK + nanase | 4.2 | 3.9 | 4.0 | |
| SV-SK-GT | 3.6 | 3.7 | 3.7 | |
| SV-SK-GT + nanase | 3.8 | 3.8 | 3.7 | |
a
Viruses passaged through human SK and SK-GT or mouse GT-KO and GT-WT cells were treated with sera as described in Materials and Methods and assayed for PFU on Vero cell monolayers. In experiment 5, viruses, where designated, were incubated 1:1 with 0.3 U of Clostridium perfringens neuraminidase (nanase) (Sigma) per ml for 1 h at 37°C before treatment with sera.