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. 2024 Apr 2;19:3123–3142. doi: 10.2147/IJN.S437714

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© 2024 Twamley et al.

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Collagen was detected on the apical surface of HUVECs. (A) CLSM images showing COL1A2 (green) and (B) COL5A2 (green) colocalization (yellow) with WGA (red) on the apical surface of formaldehyde fixed LSS- and S-cultivated HUVECs. Nuclei are shown in blue. White arrows indicate areas of colocalization (yellow) along the apical membrane. Scale bar = 5 µm. (C) CLSM image depicts COL1A2 (green) in the ESL of cells after HPF/FS. Orthogonal images display COL1A2 above the nuclei (blue) in the ESL. Inset shows TEM image of immunogold labeling for COL1A2. Clusters of gold-particles are marked by magenta circles. (D) COL5A2 (green) in the ESL of cells after HPF/FS. Orthogonal images display COL5A2 above the nuclei (blue) in the ESL. Scale bar = 10 µm. Inset shows TEM image of immunogold labeling for COL5A2. Clusters of gold-particles are marked by magenta circles. Scale bar = 1 µm.