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Collagen type V alpha 2 was detectable in mouse vessels on the apical surface of endothelial cells. CLSM detection of COL5A2 (red) on the apical surface of the endothelium in histological sections of mouse uniform flow conditioned vessels, (A) descending aorta and (B) right carotid. (C) Minor COL5A2 (red) signal was observed in the acute disturbed flow conditioned LCA. Nuclei shown in blue. Auto-fluorescence from elastin (green) was imaged to identify vessel wall. Scale Bars = 20 μm.
