TABLE 2.

NΔ450-641 inhibits replication of FR-BamHI C-Luc by wild-type EBNA-1 in 143/EBNA-1 cells

Effector	Relative efficiency of replicationa
	oriP-BamHI C-Luc	oriP-minus	FR-BamHI C-Luc	oriP-minus
Vector	100	<2.8	24 ± 6.7	<0.72
NΔ450-641	11 ± 7.2	1.1 ± 0.14	1.3 ± 0.50	<0.72

^a10 μg of each DNA was introduced into 10⁷ 143/EBNA-1 cells by electroporation. Low-molecular-weight DNA was isolated after 94 to 98 h, and the relative concentration of DpnI-resistant plasmids was determined by quantitative competitive PCR and was corrected for transfection efficiency of 143/EBNA-1 cells as described in Materials and Methods. The relative efficiency of replication by each derivative of EBNA-1 is expressed as a percentage of DpnI-resistant DNA relative to that replicated by wild-type EBNA-1, which is set to 100% (100% = 18 ± 6.5 copies of DpnI-resistant DNA per transfected cell). Data represent an average of two (for oriP-BamHI C-Luc) or four (for FR-BamHI C-Luc) experiments ± standard deviation. Data represented as “<” indicate that the level of DpnI-resistant DNA was less than the lowest concentration used for the competitor DNA and is therefore reported as less than an average of the smallest amount per transfected cell that could be determined by interpolation from the competitor DNA curve.