TABLE 3.

cis-acting sequences within a 4,877-bp fragment of FR-BamHI C-Luc substitute for the DS in short-term replication assays

Reporter	Relative efficiency of replicationa		nb
	Reporter	oriP-minus
oriP-BamHI C-Luc	100	<2.0	7
FR-BamHI C-Luc	13 ± 3.1	<0.50	8
oriP-Backbone	130 ± 115	<0.50	5
FR-Backbone	0.54 ± 0.058	<0.50	6
pΔBal 2	0.81 ± 0.27	<0.50	2
pΔBal 12	<0.50	<0.50	3
DS-BamHI C-Luc	<0.50	<0.50	5

^a10 μg of each DNA (reporter and oriP-minus DNA) was introduced into 10⁷ 143/EBNA-1 cells by electroporation. Low-molecular-weight DNA was isolated after 94 to 98 h, and the relative concentration of DpnI-resistant plasmids was determined by quantitative competitive PCR and was corrected for the transfection efficiency of 143B/EBNA-1 cells as described in Materials and Methods. The relative efficiency of replication is expressed as a percentage relative to oriP-BamHI C-Luc, which is set to 100% (100% = 26 ± 20 copies of DpnI-resistant DNA per transfected cell). Data represents an average ± standard deviation. Data represented as “<” indicate that the level of DpnI-resistant DNA was less than the smallest amount used for the competitor DNA and is therefore reported as less than an average of the smallest amount per transfected cell that could be determined by interpolation from the competitor DNA curve. 

^bn, number of times the reporter plasmid was tested during the mapping analysis.