TABLE 4.
Multiple copies of Rep* enhance short-term replication of plasmids in 143/EBNA-1 cells
| Reporter | Copies/cella | P valueb |
|---|---|---|
| oriP-Backbone | 12 ± 7.3 | 0.021 |
| FR-Backbone | <0.2c | 0.014 |
| 1862 | 1.4 ± 0.37 | |
| 1925 | 8.3 ± 6.9 | 0.021 |
| 1926 | 4.2 ± 1.1 | 0.021 |
10 μg each of reporter DNA and oriP-minus was introduced into 107 143/EBNA-1 cells by electroporation. Low-molecular-weight DNA was isolated after 94 to 98 h, and the relative concentration of DpnI-resistant plasmids was determined by quantitative competitive PCR and was corrected for transfection efficiency of 143/EBNA-1 cells as described in Materials and Methods. Data represent an average of four experiments ± standard deviation.
Data were analyzed by using the Wilcoxon rank sum test (26) with Mstat (version 1.3, by Norman Drinkwater), and the P value comparing each derivative to 1862 is noted.
The level of DpnI-resistant DNA was less than the lowest concentration used for the competitor DNA and is therefore reported as less than an average of the smallest amount per transfected cell that could be determined by interpolation from the competitor DNA curve.