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. 2024 Mar 27;121(14):e2315982121. doi: 10.1073/pnas.2315982121

Fig. 1.

Fig. 1.

LsDorsal knockdown promotes RSV replication and transmission in L. striatellus. (A and B) Detection for the transcript levels of LsDorsal injected with crude extracts of RSV-free and RSV-infected L. striatellus at various time points. (C–E) Effects of LsDorsal knockdown on the expression levels of RSV-NP in L. striatellus treated with dsLsDorsal and RSV crude extracts for transcripts at 3 and 6 dpi (C), protein at 6 dpi (D), and ratio of virus acquisition (3 dpi) and transmission (E). (F) Immunofluorescence staining of RSV NP in the SG and ovary of L. striatellus at 6 dpi after treatment with dsGFP or dsLsDorsal and RSV crude extracts. (Scale bar, 50 μm.) (G) Identification of Dorsal phosphorylation site in L. striatellus based on its homologous to Rel (Homo sapiens) and p65 (Drosophila melanogaster). (H) Phosphorylation level of Dorsal protein in the nucleus of L. striatellus at different time points after RSV infection. “VF 3 dpi” indicated that nonviruliferous L. striatellus were injected with RSV-free insect crude extracts for 3 d. “RSV 3 dpi” and “RSV 6 dpi” indicated that nonviruliferous L. striatellus were injected with RSV-infected insect crude extracts for 3 d and 6 d, respectively. H3 and GAPDH antibodies represent specific marker of the cell nucleus and cytoplasm, respectively. Three biological replicates were performed for each of the experiment (10 to 15 L. striatellus for each of the replicate). The t test method was used for significance analysis. * represents significant difference (P < 0.05), ** and *** represent extremely significant difference (P < 0.01 and P < 0.001). The error bars represent the SE of the mean.