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. 2024 Mar 27;121(14):e2315982121. doi: 10.1073/pnas.2315982121

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Copyright © 2024 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 3. — LsZN708 knockdown promotes RSV replication and transmission in L. striatellus. (A) Transcription level of LsZN708 in nonviruliferous and viruliferous L. striatellus. (B and C) Relative transcription levels of RSV-NP in viruliferous L. striatellus treated with dsGFP or dsLsZN708 at 3 dpi and 6 dpi, respectively. (D–F) Effects of dsLsZN708 knockdown on the expression levels of RSV-NP in L. striatellus treated with dsLsZN708 and RSV crude extracts for transcripts at 3 and 6 dpi (D), protein at 6 dpi (E), and ratio of virus acquisition (3 dpi) and transmission (F). (G) Immunofluorescence staining of RSV NP in the SG of L. striatellus treated with dsLsZN708 or dsLsDorsal and RSV crude extracts at 6 dpi. The scale bar represents 50 μm. Three biological replicates were conducted for each experiment. Significance was determined using the t test method. * represents significant difference (P < 0.05), ** and *** represent extremely significant difference (P < 0.01 and P < 0.001). The error bars represent the SE of the mean.