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. 2024 Mar 27;121(14):e2315982121. doi: 10.1073/pnas.2315982121

Fig. 6.

Fig. 6.

RSV NS4 competes with LsMSK2 for LsDorsal binding. (A) Interaction between RHD-n domain of LsDorsal and the C-terminus of LsMSK2 kinase confirmed through Y2H assay. (B) An in vitro pull-down assay verified the interaction between LsRHD-n and C-terminus of LsMSK2. GST-RHD-n protein were used to pull-down with His-MSK2-C. His-MSK2-C was further detected with anti-His antibody. (C) The phosphorylation of LsDorsal protein by LsMSK2 kinase protein in vitro. (D) Binding ability of LsMSK2-C to LsRHD-n with the increasing concentration of NS4 protein. (E) Binding ability of NS4 to LsRHD-n with the increasing concentration of LsMSK2-C. (F) Effect of LsMSK2 knockdown on the accumulation level of RSV in viruliferous L. striatellus treated with dsGFP and dsLsMSK2. (G) Effect of LsMSK2 knockdown on the accumulation level of RSV in L. striatellus treated with dsGFP or dsLsMSK2 and RSV crude extracts at 6 dpi. Three biological replicates were performed for each experiment. The t test method was used for significance analysis. * represents significant difference (P < 0.05), ** and *** represent extremely significant difference (P < 0.01 and P < 0.001). The error bars represent the SE of the mean.