FIG. 6.

DNA PCR analysis of sequential PBMC and plasma samples from animal 1490. Sequences in nef were amplified by nested DNA PCR using primers that span the deleted region in SIVmac239Δnef as described in Materials and Methods. (A) Sequential PBMC samples obtained before and after challenge with SIVmac251 (70*, day of challenge); (B) plasma samples from sequential time points following coculture with CEMx174 cells. M, molecular size markers; (+), amplification of nef sequences from rhesus PBMC used to propagate the SIVmac239Δnef immunization stock; (−), no DNA control.