Fig. 2. Isolation and characterization of a panel of potent neutralizing antibodies through affinity maturation.

a Neutralization profiles of the authentic henipavirus in Vero E6 cells for antibodies. The mixtures of virus and serially diluted antibodies were added to cells. After 72 h incubation, IC₅₀ were calculated by fitting the number of plaques with serially diluted antibody to a sigmoidal dose-response curve. Data are represented as the mean ± S.D. from n = 3 biologically independent experiments. b Summary of the IC₅₀ with 95% confidence intervals of antibodies evaluated by the plaque reduction neutralization titer (PRNT). >5 represents that the antibody has not reached its maximum neutralization effect at the highest dilution concentration. c Affinity measurement of antibodies for binding to immobilized NiV-RBP and HeV-RBP, measured by using BLI. The biosensors loaded with Fab fragments (NiV42 or 42-27) or RBPs were shown in Supplementary Fig. 2a. Source data are provided as a Source Data file.