Fig. 4. Somatic hypermutation in the antibody germline determines neutralizing capacities.
a Summary of the germline gene (V-D-J for heavy chain and V-J for light chain), V-gene nucleotide somatic hypermutations, and CDR3 of antibodies. The analysis of the germline nucleic acid sequence of antibodies was conducted using IMGT/V-QUEST. b Binding curve of germline-reverted antibodies to RBPs. The binding results were analyzed by fitting to a four-parameter curve using GraphPad Prism software. c Neutralization activity evolution of germline-reverted antibodies against NiV pseudovirus. The neutralization percentage of NiV pseudovirus relative to non-antibody-treated controls was determined by counting the number of GFP-positive cells 24 h postinfection. IC50 values were then calculated through nonlinear regression analysis using Prism. gHgL, VH germline paired with VL germline. gHmL, VH germline paired with antibody VL. mHgL, antibody VH paired with VL germline. The antibodies 41-6gHgL and 41-9gHgL were identical and labeled differently in the illustration to facilitate comparison. This same principle applied to 41-6mHgL and 41-9mHgL. Data were represented as the mean ± S.D from n = 3 biologically independent experiments. (b, c). Source data are provided as a Source Data file.