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. 2024 Apr 6;15:2983. doi: 10.1038/s41467-024-47275-8

Fig. 4. Oral administration of Amuc_1409 protects against irradiation-induced gut damage by accelerating ISC regeneration.

Fig. 4

a Scheme showing the experimental timeline for vehicle or Amuc_1409* (9 μg per mouse) treatment for survival rate analysis in the radiation (1100 Rad)-induced intestinal injury model. b The survival rate of vehicle- or Amuc_1409*-treated mice following 1100 Rad of radiation (n = 9 biological replicate mice per group). c Scheme showing the experimental workflow for oral administration of vehicle or Amuc_1409* (9 μg per mouse) for histological (d, e, h: n = 7 biological replicate mice per group) and qRT-PCR (f, g, i: n = 12 biological replicate mice per group) analysis of SI tissues in the radiation (1100 Rad)-induced intestinal injury model. d Representative images of H&E staining (left panel) and histological scores (right panel) in sections of SI. Scale bar, 100 μm. e Representative IF images of BrdU staining (left panel) and quantification of percentage of BrdU+ cells per crypt (right panel) in sections of SI. Scale bar, 100 μm. qRT-PCR results showing the relative mRNA expression of cell proliferation markers (Ki67, Pcna, and Ccnd1) (f) and ISC markers (Lgr5, Olfm4, Msi1, Bmi1, and Hopx) (g) in SI tissues. h Representative images of IHC staining for OLFM4 (left panel) and quantification of OLFM4+ cells per crypt (right panel) in sections of SI. Scale bar, 50 μm. i qRT-PCR results showing the relative mRNA expression of Wnt/β-catenin target genes in SI tissues. All data are presented as the mean ± SEM. Data shown in (b) and (d–i) are representative of three independent experiments, each with similar results. Each data point represents a biological replicate, corresponding to one mouse. Images in (a) and (c) were created with BioRender.com and have been granted a publication license. Statistical analyses were performed using Log-Rank test (b) and two-tailed Student’s t test (d–i) (*p < 0.05, **p < 0.01, and ***p < 0.001 vs vehicle-treated group). Source data, including the exact p values, are provided as a Source Data file.