Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Viral RNAs in infected LLC-MK2 and C6/36 cells. Viruses derived by transfection were passaged in C6/36 or in LLC-MK2 cells, as described in the legend to Fig. 6, and then used to infect cell monolayers at an MOI of 0.05. After 2 days, cells were lysed and RNA derived from one 35-mm-diameter well of a six-well plate was recovered and applied to a nylon membrane. Virus-specific RNA was detected with a 32P-labeled cDNA probe representing nt 1379 to 7871 of the DEN2 genome.
