FIG. 2.

Vpr induces apoptosis during single-cycle infection of dividing Jurkat T cells. (A) Jurkat T cells were infected with VSV-G pseudotyped Vpr⁺ or Vpr⁻ HIV-1 viruses. At 48 h p.i., the infected or mock-infected cells were costained with Annexin V and PI and analyzed by flow cytometry. The axes represent the cell-associated fluorescence intensity of Annexin V (x axis) and PI (y axis). (B) The percentage of Annexin-V⁺ PI⁺ or Annexin-V⁺ PI⁻ cells within the different infected cell populations were evaluated by Annexin V-PI costaining and flow cytometry analysis at 1, 2, and 3 days p.i.