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. 2024 Mar 26;18(1):wrae048. doi: 10.1093/ismejo/wrae048

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© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Anatomy features of Catillopecten margaritatus and photomicrographs showing the extracellular localization of the SOB. A: Anatomy features of C. margaritatus showing the gill tissue, left valve. B-D: Hematoxylin–eosin (HE) stain of gill sections showing a gross view of the gill filaments. E-H: Transmission electron microscopy (TEM) of gill filament showing the extracellular distribution of the SOBs. The bacteria are in the extracellular spaces filled by microvilli, indicated by arrows. Scale bar: A: 1 mm; B: 50 μm; C-D: 10 μm; E: 10 μm; F: 5 μm; G: 2 μm; H: 1 μm. Am, adductor muscle; b: Bacteria; dg, digestive gland; e: Endocytosis; f, foot; go, gonad; gi, gill; ma, mantle; mi: Microvillus; t, tentacle.