TABLE 2.
Competition of HMAbs for CG10 binding to the gp120 glycoproteina
| BH10 gp120 | HMAb (10 μg/ml) | sCD4 (1 μg/ml) | CG10 bound |
|---|---|---|---|
| − | None | − | 0.057 ± 0.017 |
| + | None | − | 0.083 ± 0.014 |
| + | None | + | 0.324 ± 0.053 |
| + | C11 | + | 0.324 ± 0.019 |
| + | 2G12 | + | 0.354 ± 0.023 |
| + | 212A | + | 0.303 ± 0.045 |
| + | 15e | + | 0.117 ± 0.013 |
| + | 48d | + | 0.063 ± 0.012 |
| + | 17b | + | 0.053 ± 0.007 |
| + | A32 | + | 0.583 ± 0.016 |
a
Binding of the CG10 antibody to the BH10 gp120 glycoprotein was measured in an ELISA format. The gp120 glycoprotein was captured on ELISA plates with an antibody (D7324) that binds to the C terminus of gp120. Values expressed are means ± standard deviations for replicate wells.