FIG. 4.

Expression of alkaline phosphatase after secondary Ad.CMV-AlkPhos administration to mice treated with the combined regimen at the time of primary vector administration. Results are the mean ± SD of the numerical score for expression based on a scale of 0 to 5. Expression in the bronchial epithelium is shown on the left, and expression in the alveolar epithelium is shown on the right. Data were derived from two experiments with three to five mice per time point per group, with the exception of the 21- to 24-day combined treatment group (two mice). The primary controls had not previously received Ad vector. The other groups received either L6 or no treatment (secondary control) or the combined regimen (secondary combined) on days −1, 2, and 7 relative to the time of primary Ad vector administration 98 to 115 days earlier. ∗, P < 0.05 with respect to the other two groups at that time point.