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Creating point patterns and density kernels
(A) Staining of NeuN and GFAP intended for point patterns transformation.
(B) The xy coordinates of detected cells in QuPath are transformed to point patterns using the ppp function from spatstat. A convex hull is applied to restrict the observation window.
(C) Different sigma levels from the spatstat’s density function provide alternative mapping resolution (density kernels) for the cells of interest.
