Table I.
MRM transitions and specific parameters for the targeted peptides, FTFSLDTSK, VLIYFTSSLHSGVPSR and STAYLQMNSLR, and IS P14R.
| Selected peptide | Region | MRM transition, m/z | Q1 Pre Bias, V | CE, V | Q3 Pre Bias, V |
|---|---|---|---|---|---|
| FTFSLDTSK | H-chain of | 523.30([M+2H]2+)→797.40(y7+)a | −38 | −18 | −34 |
| CDR2 | 523.30([M+2H]2+)→898.50(y8+) | −38 | −20 | −30 | |
| 523.30([M+2H]2+)→650.30(y6+) | −38 | −19 | −34 | ||
| VLIYFTSSLHSGVPSR | L-chain of | 588.50([M+3H]3+)→776.10(y14++) | −30 | −19 | −32 |
| CDR2 | 588.50([M+3H]3+)→939.60(y9+) | −22 | −28 | −26 | |
| 588.50([M+3H]3+)→602.40(y6+) | −22 | −28 | −24 | ||
| STAYLQMNSLR | H-chain of | 642.60([M+2H]2+)→861.30(y7+) | −32 | −23 | −28 |
| CH1 | 642.60([M+2H]2+)→748.30(y7+) | −24 | −22 | −24 | |
| 642.60([M+2H]2+)→620.30(y7+) | −34 | −24 | −24 | ||
| P14R (IS) | - | 512.10→292.30a | −38 | −20 | −20 |
| 512.10→389.30 | −38 | −16 | −28 | ||
| 512.10→757.50 | −38 | −19 | −38 |
a
Quantitation peptides. IS, internal standard; P14R, Pro14-Arg; MRM, multiple reaction monitoring; Q, quadrupole; CE is short for collision energy; V means voltage.